Effect of lipopolysaccharide (LPS) from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica

Responses of Fasciola hepatica infected sheep to various infection levels.

The response to Fasciola hepatica was studied in sheep infected with 5, 30, 150 metacercariae. The animals were necropsied 12 weeks post-infection (p-i) for counting and measuring flukes. Cellular and humoral responses were detected by peripheral eosinophil count, peripheral blood lymphocyte proliferation with excretory-secretory products (FhESP) and ELISA. All sheep were infected at necropsy e...

Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine proteinases of adult fluke.

The use of cysteine proteinases from Fasciola hepatica adult flukes for the serodiagnosis of caprine fasciolosis by means of an indirect ELISA test was studied. Two proteolytic fractions from adult fluke homogenates, with apparent molecular weights of 28 and 34 kDa (P28 and P34 respectively), were characterised as cysteine proteinases using azocasein assays and gelatin gel analysis. Both P28 an...

Ion transport in the colon of rats experimentally infected with liver fluke (Fasciola hepatica).

The effect of liver fluke infection (Fasciola hepatica) on ion transport in the rat proximal colon was evaluated with electrophysiological methods using an Ussing apparatus. Rats were orally infected with F. hepatica metacercariae. The experimental groups of rats at 4 weeks post-infection (wpi) were in the acute stage of fasciolosis, while those at 7, 10 and 13 wpi represented the chronic phase...

The efficiency of diamphenethide against Fasciola hepatica in sheep

Fasciola hepatica: comparative metacercarial productions in experimentally-infected Galba truncatula and Pseudosuccinea columella

As large numbers of metacercariae of Fasciola hepatica are necessary for research, experimental infections of Galba truncatula and Pseudosuccinea columella with this digenean were carried out to determine the better intermediate host for metacercarial production and, consequently, the most profitable snail for decreasing the cost price of these larvae. Pre-adult snails (4 mm in shell height) or...